Journal: Frontiers in Cellular Neuroscience

Article Title: Platelet Activating Factor Enhances Synaptic Vesicle Exocytosis Via PKC, Elevated Intracellular Calcium, and Modulation of Synapsin 1 Dynamics and Phosphorylation

doi: 10.3389/fncel.2015.00505

Figure Lengend Snippet: The PAFR co-localizes to synapses. (A,B) Immunofluorescence of hippocampal neuronal cultures 21 days in vivo (DIV). PAFR (green); presynaptic marker vGlut (blue); postsynaptic marker PSD95 (red). (A) Boxed region is shown with 3× magnification in left corner and further magnified in (B) . Scale bars A = 20 μm, B = 3 μm. Arrows highlight PAFR puncta touching vGlut+/PSD95+ synapses. (C) Colocalization of PAFR, PSD95, and vGlut calculated as Pearsons correlation coefficient. (D) Quantification of the percent of PSD95 or vGlut puncta touching PAFR puncta. (E) Quantification of the percent of PAFR puncta that contact vGut+/PSD95+ synapses and the percent of vGlut+/PSD95+ synapses that contact PAFR puncta. Error bars represent ±SEM. (F) Immunofluorescence of hippocampal neuronal cultures. PAFR (green); inhibitory presynaptic marker vGAT (red); inhibitory postsynaptic marker Gephrin (blue). Scale bar = 3μm. Arrows highlight PAFR puncta touching vGAT+/Gephrin+ synapses.

Article Snippet: Cells were permeabilized with 0.1% triton-X (Promega) in PBS for 5 min. After blocking with 4% normal goat serum (Vector Laboratories) in PBS for 30 min, cells were incubated for 1.5 h using these primary antibodies: PAFR (Bioss bs-1478R), vGlut (EMD Millipore #5905), PSD95 (NeuroMAB clone K28/43 cat#75-028), vGAT (Synaptic Systems #131 004), and Gephrin (Synaptic Systems #147 021) in blocking buffer.

Techniques: Immunofluorescence, In Vivo, Marker

Journal: Cell reports

Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species

doi: 10.1016/j.celrep.2023.113151

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Gephryin antibody , Synaptic Systems , Cat. # 147 318; RRID:AB_2661777.

Techniques: Virus, Recombinant, Transfection, Magnetic Beads, Luciferase, RNAscope, Multiplex Assay, Reverse Transcription, Control, Plasmid Preparation, Software